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Flow Cytometry of Hematological Malignancies contains an array of graphical outputs produced by the technique in the study of the most (and the least) common diseases. The images included allow you to compare your own results with a third party reference pattern.   There is a detailed description of the main leukocyte antigens, together with a description of their distribution amongst normal and abnormal blood cells. The book also provides a comprehensive description of the phenotype of every neoplastic blood disease recorded in the WHO classification system, including all the instructions needed to recognise and classify even the least common entity.

Designed to be practical, the book is perfect for quickconsultation and is divided into two main sections. Section I dealswith the direct object of immunophenotyping, and Section II dealswith the ultimate target of the analysis. More than 50 antigens arecovered and every antigen is dealt with in three main parts: general features, cytometric features and practical hints.

This authoritative and state-of-the-art reference will beinvaluable for clinicians directly involved in the diagnosis andanalysis of hematological diseases, including hematologists, hematopathologists, oncologists, pathologists and techniciansworking in diagnostic laboratories.


This book is the updated English version of the 2006 German bestseller 'Zelluläre Diagnostik', a comprehensive presentation of flow cytometry and its applications. While some techniques of immunophenotyping by flow cytometry already are routine procedures in the laboratory, new methods for the functional characterization of cells, the analysis of rare cells, and the diagnosis of complex materials have only begun to win wide recognition. New approaches such as slide-based cytometry will lead to an increase in the use of cytometric techniques. Multiparameter approaches will further improve analysis. The book provides a comprehensive and detailed compilation of all aspects of flow cytometry in research and the clinic. For newcomers it offers a thorough introduction, for advanced users, specific protocols and interpretation assistance.

Editors: Ulrich Sack, Attila Tárnok, Gregor Rothe


  1. Richards SJ. Introduction to ICCS/ESCCA consensus guidelines to detect GPI-deficient cells in paroxysmal nocturnal hemoglobinuria and related disorders. Cytometry B Clin Cytom. 2018 Jan;94(1):12-13. doi: 10.1002/cyto.b.21617. PubMed PMID: 29389086.

  2. Preffer F. Special Issue: Updated ICCS/ESCCA PNH guidelines. Cytometry B Clin Cytom. 2018 Jan;94(1):11. doi: 10.1002/cyto.b.21616. PubMed PMID: 29389084.

  3. Oldaker T, Whitby L, Saber M, Holden J, Wallace PK, Litwin V. ICCS/ESCCA consensus guidelines to detect GPI-deficient cells in paroxysmal nocturnal hemoglobinuria (PNH) and related disorders part 4 - assay validation and quality assurance. Cytometry B Clin Cytom. 2018 Jan;94(1):67-81. doi: 10.1002/cyto.b.21615. PubMed PMID: 29251828.

  4. Sutherland DR, Illingworth A, Marinov I, Ortiz F, Andreasen J, Payne D, Wallace PK, Keeney M. ICCS/ESCCA consensus guidelines to detect GPI-deficient cells in paroxysmal nocturnal hemoglobinuria (PNH) and related disorders part 2 - reagent selection and assay optimization for high-sensitivity testing. Cytometry B Clin Cytom. 2018 Jan;94(1):23-48. doi: 10.1002/cyto.b.21610. PubMed PMID: 29236353.

  5. Dezern AE, Borowitz MJ. ICCS/ESCCA consensus guidelines to detect GPI-deficient cells in paroxysmal nocturnal hemoglobinuria (PNH) and related disorders part 1 - clinical utility. Cytometry B Clin Cytom. 2018 Jan;94(1):16-22. doi: 10.1002/cyto.b.21608. PubMed PMID: 29236352.

  6. Illingworth A, Marinov I, Sutherland DR, Wagner-Ballon O, DelVecchio L. ICCS/ESCCA consensus guidelines to detect GPI-deficient cells in paroxysmal nocturnal hemoglobinuria (PNH) and related disorders part 3 - data analysis, reporting and case studies. Cytometry B Clin Cytom. 2018 Jan;94(1):49-66. doi: 10.1002/cyto.b.21609. PubMed PMID: 29236350.

  7. Rawstron AC, Kreuzer KA, Soosapilla A, Spacek M, Stehlikova O, Gambell P, McIver-Brown N, Villamor N, Psarra K, Arroz M, Milani R, de la Serna J, Cedena MT, Jaksic O, Nomdedeu J, Moreno C, Rigolin GM, Cuneo A, Johansen P, Johnsen HE, Rosenquist R, Niemann CU, Kern W, Westerman D, Trneny M, Mulligan S, Doubek M, Pospisilova S, Hillmen P, Oscier D, Hallek M, Ghia P, Montserrat E. Reproducible diagnosis of chronic lymphocytic leukemia by flow cytometry: An European Research Initiative on CLL (ERIC) & European Society for Clinical Cell Analysis (ESCCA) Harmonisation project. Cytometry B Clin Cytom. 2018 Jan;94(1):121-128. doi:10.1002/cyto.b.21595. Epub 2018 Jan 17. PubMed PMID: 29024461; PubMed Central PMCID: PMC5817234.

  8. Lambert C, Preijers FWMB, Yanikkaya Demirel G, Sack U. Monocytes and macrophages in flow: an ESCCA initiative on advanced analyses of monocyte lineage using flow cytometry. Cytometry B Clin Cytom. 2017 May;92(3):180-188. doi:10.1002/cyto.b.21280. Epub 2015 Dec 12. PubMed PMID: 26332381. 

  9. Rawstron AC, Paiva B, Stetler-Stevenson M. Assessment of minimal residual disease in myeloma and the need for a consensus approach. Cytometry B Clin Cytom. 2016 Jan;90(1):21-5. doi: 10.1002/cyto.b.21272. Epub 2015 Sep 23. PubMed PMID: 26202864.

ESCCA is grateful for its sponsors
Sysmex sponsor
Sponsor Beckman Coulter


European Society for Clinical Cell Analysis (ESCCA)
p/a Kinderdijkstraat 14 II
1079 GH Amsterdam
The Netherlands

Correspondence to

Prof. Silvia Della Bella, ESCCA Secretary

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