Flow Cytometry of Hematological Malignancies [Hardcover] 
Claudio Ortolani (Author)

it was released on 15th July 2011.


Cellular Diagnostics-basic principles, 
methods and applications of flow cytometry

Editors: Ulrich Sack, Attila Tárnok, Gregor Rothe



MC Bene et al. Immunophenotyping of acute leukaemia and lymphoproliferative disorders: a consensus proposal of the European LeukemiaNet Work Package 10. Leukemia (2011) 25: 567-574.
The title says it all. This is a multicenter comprehensive report with a description of the mandatory as well as complementary markers that should be used in each condition. Anyone working with leukemias and lymphomas should go through it.


Z Darzynkiewicz et al. Cytometry of DNA replication and RNA synthesis: Historical perspective and recent advances based on “click Chemistry” . Cytometry part A (2011) 79A(5): 328-337.
Again the title says it all. We all know that before the “HIV era”, that led to the development of modern time flow cytometers, a very important area of applications was “DNA analysis by flow cytometry”. It is still very important and in this paper written by a pioneer in the field, the past, the present and the future are described.


Daniel Grey, Ulrich Sack et al. Increased CD64 expression on polymorphonuclear Neutrophils indicates infectious complications following solid organ transplantation. Cytometry part A (2011) 79A(6): 446-460.
Here is an easy, non invasive way to distinguish infectious complications following solid organ transplantation. This paper provides also a lot of information regarding follow-up of these patients by flow cytometry.


Preijers FW , Huys E , Leenders M , Nieto L , Gautherot E , Moshaver B The new violet laser dye, Krome Orange, allows an optimal polychromatic immunophenotyping based on CD45-KO gating. "Journal of immunological methods" J Immunol Methods. 2011 Sep 30;372(1-2):42-51. Epub 2011 Jul 14. 
A very useful solution to the problems of polychromatic clinical cytometry.


Venetia Bigley and Matthew Collin Dendritic cell, monocyte, B and NK lymphoid deficiency defines the lost lineages of a new GATA-2 dependent myelodysplastic syndrome. Haematologica, 2011 96(8)-1081
A publication of much interest for haem-onc diagnostics flow cytometrists.